| Abstract: | 衛福部國民健康署統計資料顯示，大腸癌是台灣癌症發生人數最多的癌症，每年約有1萬多人得到結腸直腸癌，其中約五分之一的病患為第四期。癌細胞的侵襲及轉移往往是癌症病患致死且導致預後不良的主要原因之一，而其侵入則與細胞間質的分解、細胞的貼附能力及移動性的改變有關。有研究發現，舞菇的子實體及菌絲體均含有豐富的營養成分，其對糖尿病、高血壓、高血脂等有明顯的治療效果，其中舞菇含有的多醣體在腸胃中具有提升免疫力和抗腫瘤效果。本實驗使用灰、白兩種舞菇水萃取物處理具有侵襲能力的人類結腸直腸癌細胞株LoVo，分別先進行24、48小時MTT assay，並從中找到安全劑量，以wound healing assay及transwell migration等實驗評估舞菇水萃取物抑制結腸直腸癌細胞轉移的能力。實驗結果顯示，LoVo細胞在經過不同劑量下的灰、白舞菇水粗萃物並培養24、48小時後，隨著萃取物的濃度增加，LoVo細胞的存活率明顯下降，也得知兩種舞菇水萃取物的安全濃度為0.8 mg/mL以下。在wound healing assay及transwell migration方面，兩種舞菇萃取物分別處理48小時後皆呈現劑量效應，而且兩種舞菇水萃取物顯著抑制細胞爬行距離以及transwell migration程度。經由western blot分析，也發現灰、白舞菇水萃取物可以減少誘發EMT過程之相關因子N-cadherin、β-catenin、Vimentin、Snail及Slug的蛋白質表現量。由以上實驗結果可以得知，低濃度之灰、白舞菇水萃取物能有效抑制LoVo轉移的功能，可能具有輔助治療大腸癌轉移之能力。
According to statistics from the Health promotion Administration, Ministry of Health and Welfare, colorectal cancer is the most commonly occurring cancer in Taiwan. About 10,000 people get colorectal cancer each year, and about one in five patients are in the fourth phase. Invasion and metastasis of cancer cells are often one of the major causes of cancer death and lead to poor prognosis. Invasion is related to the breakdown of cell stroma, cell attachment and mobility changes. Studies have found that fruiting bodies of Grifola frondosa and mycelium are rich in nutrients, which have significant effects on diabetes, hypertension, hyperlipidemia, etc., in which the polysaccharide contained in Grifola frondosa has the immunity in the stomach and anti-tumor effect. In this experiment, grey and white Grifola frondosa water extracts with invasive human colorectal cancer cell line LoVo, respectively, 24,48 hours MTT assay and find a safe dose to wound healing assay and transwell migration two an experiment was conducted to evaluate the ability of Grifola frondosa water extracts to inhibit the metastasis of colorectal cancer cells. The experimental results showed that after LoVo cells were cultured for 24,48 hours under different doses of grey and white Grifola frondosa water extracts, the survival rate of LoVo cells decreased significantly with the increase of the extract content, The Grifola frondosa water extracts safe concentration of 0.8 mg / mL or less. In the wound healing assay and transwell migration, the two kinds of Grifola frondosa water extracts were treated for 48 hours, respectively, showing dose-effect, and two kinds of Grifola frondosa water extracts significantly inhibited cell creep distance and transwell migration degree. Western blot analysis also showed that Grifola frondosa water extracts can reduce the protein expression of N-cadherin, β-catenin, Vimentin, Snail and Slug, which are involved in the process of EMT. From the above experimental results we can see that the low concentration of grey and white Grifola frondosa water extracts rough extract effectively inhibit the function of LoVo metastasis may have the ability to assist the treatment of metastatic colorectal cancer. |
