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http://asiair.asia.edu.tw/ir/handle/310904400/110725
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| Title: | Phenethyl Isothiocyanate (PEITC) and Benzyl Isothiocyanate (BITC) Inhibit Human Melanoma A375.S2 Cell Migration and Invasion by Affecting MAPK Signaling Pathway In Vitro |
| Authors: | MA, YI-SHIH;HS, YUNG-TING;HSIAO, YUNG-TING;LIN, JEN-JYH;CHING-LUNG, LUNG L;LIAO, CHING-LUNG;CHIN-CHUNG, L;LIN, CHIN-CHUNG |
| Contributors: | 生物科技學系 |
| Date: | 2017-11 |
| Issue Date: | 2018-04-03 01:14:31 (UTC+0) |
| Abstract: | Background/Aim: Numerous evidence has shown
that PEITC and BITC inhibit cancer cell migration and
invasion. In this study, we investigated the anti-metastatic
mechanisms of PEITC and BITC in human melanoma cancer
A375.S2 cells in vitro. Materials and Methods: We used a
cell viability assay, an in-vitro scratch wound healing assay,
a transwell assay for cell migration and invasion, a gelatin
zymography assay, western blotting and EMSA to examine
the anti-metastatic mechanisms of PEITC and BITC in
A375.S2 cells. Results: Sublethal concentrations of PEITC
(0, 1, 2 and 2.5 μM) and BITC (0, 0.5, 1 and 2 μM) inhibited
mobility, migration and invasion of A375.S2 cells that were
assayed by wound healing and Transwell filter. PEITC and
BITC inhibited MMP-2 activity in A375.S2 cells, as assessed
by gelatin zymography assay. Results from western blotting
indicated that PEITC (2.5 μM) and BITC (2 μM) decreased
the levels of p-p38 following 24 and 48 h treatment. PEITC
(1-2.5 μM) reduced the levels of p-JNK1/2 proteins following
48-h treatment but BITC increased p-JNK1/2 levels following
24-h treatment. PEITC (2.5 μM) reduced the levels of pERK1/2
proteins following 48-h treatment but BITC (0.5-2
μM) increased p-ERK1/2 levels following 24- and 48-h
treatment. PEITC and BITC affect cell migration and
invasion of A375.S2 cells via MAPK pathway. PEITC and
BITC inhibited MMP-2 activity. PEITC increased NF-ĸB
expression but BITC decreased NF-ĸB expression in the
nucleus. Furthermore, NF-ĸB p65 binding to DNA was
decreased following 2.5 μM PEITC treatment, but increased
following treatment with 1-2 μM. However, 0.5-2 μM BITC
treatment decreased the binding of NF-ĸB to DNA in
A375.S2 cells, as assessed by electrophoretic mobility shift
(EMSA) assay. Conclusion: Based on these observations, we
suggest that PEITC and BITC can be used as antimetastastic
agents of human melanoma cells in the future. |
| Relation: | ANTICANCER RESEARCH |
| Appears in Collections: | [生物科技學系] 期刊論文
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