Bufalin induced apoptosis in SCC?4 human tongue cancer cells by decreasing Bcl?2 and increasing Bax expression via the mitochondria?dependent pathway

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題名:	Bufalin induced apoptosis in SCC?4 human tongue cancer cells by decreasing Bcl?2 and increasing Bax expression via the mitochondria?dependent pathway
作者:	CHOU, HAN-YU;MA, YI-SHIH;RICK, SAI-CHU;WU, RICK SAI-CHUEN;CHING-LUNG, LUNG L;LIAO, CHING-LUNG;CHU, YUNG-LIN;范宗宸;Fan, Chung-Chen;HUAN, WEN-WEN;HUANG, WEN-WEN
貢獻者:	生物科技學系
日期:	2017-12
上傳時間:	2018-04-03 01:13:43 (UTC+0)
摘要:	The aim of the present study was to investigate the cytotoxic effects of bufalin on SCC-4 human tongue cancer cells. Cell morphological changes and viability were examined using phase contrast microscopy and flow cytometry, respectively. The results indicated that bufalin induced morphological changes and reduced total viable cells. Apoptotic cell death was analyzed by DAPI staining and DNA gel electrophoresis; the results revealed that bufalin induced cell apoptosis. Levels of reactive oxygen species (ROS), Ca2+, nitric oxide (NO) and mitochondrial membrane potential (ΔΨm) were measured by flow cytometry, and bufalin was observed to increase Ca2+ and NO production, decrease the ΔΨm and reduce ROS production in SCC-4 cells. In addition, western blotting was performed to detect apoptosis-associated protein expression. The results demonstrated that bufalin reduced the expression of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and increased the expression of the pro-apoptotic protein, Bcl-2-associated X protein. However, bufalin treatment also increased the expression of other apoptosis-associated proteins such as apoptosis-inducing factor and endonuclease G in SCC-4 cells. Based on these findings, bufalin may induce apoptotic cell death via mitochondria-dependent pathways in human tongue cancer SCC-4 cells.
關聯:	Molecular Medicine Reports
顯示於類別:	[生物科技學系] 期刊論文

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