Aromatherapy is widely used for relaxation. This research is to focus on autonomic nervous system (ANS) variation due to aromatherapy. The subjects were 54 teachers within three elementary schools (asthma, hypertension, hypotension and heart attack disease patients are excluded). Atomization Bergamot oil was used for 10 minutes. Biosignals, such as SYS, DIA, HR, HRV, LF%, HF%, LF/HF were collected before and after aromatherapy with the aid of ANSWatch� monitor. BAI-II (Beck Anxiety Inventory) survey was also used as indicators. Result demonstrates that there were decreasing for SYS, DIA , HR and LF% and LF/HF; while there were increasing for HRV and HF% after using Aromatherapy (p<0.05). Further investigation with groups by position, age and sex variable also showed that there was significant difference with aroma treatment. The only insignificant outcome group was subjects with light anxious degree and substitute teachers. This study is the first article to investigate aromatherapy performance for elementary faculty in Taiwan. The outcome shows that aromatherapy is effective to enhance parasympathetic activation and to reduce blood pressure. Aromatherapy may be good choice for relaxation in high working pressure workers, such as elementary school faculty.