Antrodia salmonea induces G2 cell-cycle arrest in human triple-negative breast cancer ；MDA-MB-231 cells and suppresses tumor growth in athymic nude mice

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Title:	Antrodia salmonea induces G2 cell-cycle arrest in human triple-negative breast cancer ；MDA-MB-231 cells and suppresses tumor growth in athymic nude mice
Authors:	Yang, 張嘉婷；Chia-Ting Chang；許游章；Hseu You-Cheng；Varadharajan；Varadharajan Thiyagarajan；Hui-Chi Huan；Hui-Chi Huang；Li-Sung Hsu；Li-Sung Hsu；黃佩珍；Pei-Jane Huang；Jer-Yuh Liu；Jer-Yuh Liu；Jiunn-Wang L；Jiunn-Wang Liao；Hsin-Ling Ya；Hsin-Ling
Contributors:	保健營養生技學系
Date:	2017-01
Issue Date:	2017-12-08 06:10:54 (UTC+0)
Abstract:	Ethnopharmacological relevance Antrodia salmonea (AS), is a well-known folk medicinal mushroom in Taiwan, has been reported to exhibit anti-oxidant, anti-angiogenic, and anti-inflammatory effects. Materials and methods In the present study, we examined the effects of AS on cell-cycle arrest in vitro in MDA-MB-231 cells and on tumor regression in vivo using an athymic nude mice model. Results AS (0–200 μg/mL) treatment significantly induced G2 cell-cycle arrest in MDA-MB-231 cells by reducing the levels of cyclin B1, cyclin A, cyclin E, and CDC2 proteins. In addition, N-acetylcysteine (NAC) pretreatment prevented AS induced G2 cell-cycle arrest, indicating that ROS accumulation and subsequent cell cycle arrest might be a major mechanism of AS-induced cytotoxicity. Further, AS treatment decreased COX-2 expression and induced PARP cleavage was significantly reversed by NAC pretreatment in MDA-MB-231 cells. The in vivo study results revealed that AS treatment was effective in terms of delaying the tumor incidence and reducing the tumor growth in MDA-MB-231-xenografted nude mice. TUNEL assay, immunohistochemical staining and Western blotting confirmed that AS significantly modulated the xenografted tumor progression as demonstrated by induction of apoptosis, autophagy, and cell-cycle arrest. Conclusion Our data strongly suggest that Antrodia salmonea could be an anti-cancer agent for human breast cancer.
Relation:	JOURNAL OF ETHNOPHARMACOLOGY
Appears in Collections:	[Department of Food Nutrition and Healthy Biotechnology] Journal Article

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