WISP-1 positively regulates angiogenesis by controlling VEGF-A expression in human osteosarcoma

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/108150

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题名:	WISP-1 positively regulates angiogenesis by controlling VEGF-A expression in human osteosarcoma
作者:	Tsai HC；Tsai HC；Tzeng HE；Tzeng HE；Huang CY；Huang CY；黃元勵；HUANG, YUAN-LI；Tsai CH；Tsai CH；Wang SW；Wang SW；Wang PC；Wang PC；Chang AC；Chang AC；Fong YC；Fong YC；Tang CH；Tang CH
贡献者:	生物科技學系
日期:	2017
上传时间:	2017-10-30 02:41:28 (UTC+0)
摘要:	In recent years, much research has focused on the role of angiogenesis in osteosarcoma, which occurs predominantly in adolescents and young adults. The vascular endothelial growth factor-A (VEGF-A) pathway is the key regulator of angiogenesis and in osteosarcoma. VEGF-A expression has been recognized as a prognostic marker in angiogenesis. Aberrant WNT1-inducible signaling pathway protein-1 (WISP-1) expression is associated with various cancers. However, the function of WISP-1 in osteosarcoma angiogenesis is poorly understood. We demonstrate a positive correlation between WISP-1 and VEGF-A expression in human osteosarcoma. Moreover, we show that WISP-1 promotes VEGF-A expression in human osteosarcoma cells, subsequently inducing human endothelial progenitor cell (EPC) migration and tube formation. The focal adhesion kinase (FAK), Jun amino-terminal kinase (JNK), and hypoxia-inducible factor (HIF)-1α signaling pathways were activated after WISP-1 stimulation, while FAK, JNK, and HIF-1α inhibitors or small interfering RNA (siRNA) abolished WISP-1-induced VEGF-A expression and angiogenesis. In vitro and in vivo studies revealed down-regulation of microRNA-381 (miR-381) in WISP-1-induced VEGF-A expression and angiogenesis. Our findings reveal that WISP-1 enhances VEGF-A expression and angiogenesis through the FAK/JNK/HIF-1α signaling pathways, as well as via down-regulation of miR-381 expression. WISP-1 may be a promising target in osteosarcoma angiogenesis.
關聯:	Cell Death & Disease
显示于类别:	[生物科技學系] 期刊論文

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