WISP-1 positively regulates angiogenesis by controlling VEGF-A expression in human osteosarcoma

ASIAIR > College of Medical and Health Science > Department of Biotechnology > Journal Article > Item 310904400/108148

Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/108148

Title:	WISP-1 positively regulates angiogenesis by controlling VEGF-A expression in human osteosarcoma
Authors:	蔡筱琪;Hsiao-Chi Tsai;Huey-En Tzen;Huey-En Tzeng;Chun-Yin Hua;Chun-Yin Huang;黃元勵;HUANG, YUAN-LI;Chun-Hao Tsa;Chun-Hao Tsai;Shih-Wei Wan;Shih-Wei Wang;Po-Chuan Wan;Po-Chuan Wang;An-Chen Chan;An-Chen Chang;Yi-Chin Fong;湯智昕;Chih-Hsin Tang
Contributors:	生物科技學系
Date:	2017-04
Issue Date:	2017-10-30 02:41:17 (UTC+0)
Abstract:	In recent years, much research has focused on the role of angiogenesis in osteosarcoma, which occurs predominantly in adolescents and young adults. The vascular endothelial growth factor-A (VEGF-A) pathway is the key regulator of angiogenesis and in osteosarcoma. VEGF-A expression has been recognized as a prognostic marker in angiogenesis. Aberrant WNT1-inducible signaling pathway protein-1 (WISP-1) expression is associated with various cancers. However, the function of WISP-1 in osteosarcoma angiogenesis is poorly understood. We demonstrate a positive correlation between WISP-1 and VEGF-A expression in human osteosarcoma. Moreover, we show that WISP-1 promotes VEGF-A expression in human osteosarcoma cells, subsequently inducing human endothelial progenitor cell (EPC) migration and tube formation. The focal adhesion kinase (FAK), Jun amino-terminal kinase (JNK), and hypoxia-inducible factor (HIF)-1α signaling pathways were activated after WISP-1 stimulation, while FAK, JNK, and HIF-1α inhibitors or small interfering RNA (siRNA) abolished WISP-1-induced VEGF-A expression and angiogenesis. In vitro and in vivo studies revealed down-regulation of microRNA-381 (miR-381) in WISP-1-induced VEGF-A expression and angiogenesis. Our findings reveal that WISP-1 enhances VEGF-A expression and angiogenesis through the FAK/JNK/HIF-1α signaling pathways, as well as via down-regulation of miR-381 expression. WISP-1 may be a promising target in osteosarcoma angiogenesis.
Relation:	Cell Death & Disease
Appears in Collections:	[Department of Biotechnology] Journal Article

Files in This Item:

File	Size	Format
index.html	0Kb	HTML	344	View/Open

Loading...