Cultivation Mode of Flammulina velutipes to Improve Ergothioneine Content and Development of Novel Solid State Fermentation of Cordyceps militaris

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Title:	Cultivation Mode of Flammulina velutipes to Improve Ergothioneine Content and Development of Novel Solid State Fermentation of Cordyceps militaris
Authors:	Lin, Shu-Han
Contributors:	保健營養生技學系
Keywords:	Flammulina velutipes;Cordyceps militaris;Ergothioneine;Histidine;Ergosterol;Anti-oxidative
Date:	2017
Issue Date:	2017-09-15
Publisher:	亞洲大學
Abstract:	Edible and medicinal mushrooms contains many biologically active compounds such as polysaccharides, triterpenes, ergothinoneine and ergosterol. Cordyceps militaris always hears traditional Chinese medicine, also has a variety of biological activity. Both are provide anti-tumor, lowering blood pressure and sugar, lowering cholesterol, osteoporosis and other health effects. In this study, the optimum conditions cultivation process of Flammulina velutipes add Pennisetum alopecuroides and histidine. Solid state fermentation of Cordyceps militaris add Pennisetum alopecuroides. To explore its physiological activity components, antioxidant components and the impact of its nature. Ergothioneine is a kind of precious amino acid, which exist in plants and animal tissues, it can not be biosynthesized in human, only can be absorbed from the diet. The result showed that the ergothioneine contents of add to 0, 0.5, 1 and 2% histidine from fruit body were 252.61, 233.16, 292.11 and 274.49 µg/g dry weight, respectively. The ergothioneine contents of medium add 1% histidine were 219.68 µg/g and spray 0.5, 1 and 2% histidine from mushroom buds were 224.44 and 219.68 µg/g dry weight. The polysaccharide content of medium add 1% histidine (84.99 mg/g) was the highest, the second is spray 1% histidine from mushroom buds were 82.55 mg/g. The cultivation substrate add 2% histidine (62.37 mg/g) was the lowest. Pertaining to antioxidant activity (EC50 value) of Flammulina velutipes, the result showed that the scavenging ability on DPPH radicals of the cultivation substrate alternative 25% sawdust was best. With regard to chelating abilities on ferrous ions, the chelating ability of cultivation substrate add 2% histidine was best. The reducing power of add 0% histindine, medium add 1% histidine, spray 0.5 and 1% histidine were the best. On anti-oxidative components, the total phenol content of spray 1% histidine from mushroom buds (7.37 mg/g) is the first, the second is medium add 1% histidine (7.13 mg/g). The flavones content of spray 1% histidine from mushroom buds (2.10 mg/g) is the first, the second is medium add 1% histidine (1.64 mg/g). In general, add histidine can promote the function. Physiological analysis of the results show that the ergosterol contents of the cultivation substrate by Chenopodium quinoa (48.55 µg/g) and the cultivation substrate by Chenopodium quinoa add Pennisetum alopecuroides (236.68 µg/g) is the best. The cordycepin and adenosine contents of the by Chenopodium quinoa were 915.74 and 657.35 µg/g, the cultivation substrate by Chenopodium quinoa add Pennisetum alopecuroides were 2753.25 and 638.01 µg/g is the best. Overall, cultivation process of Flammulina velutipes add histidine can promote the function. The ergosterol, cordycepin and adenosine contents of Solid state fermentation of Cordyceps militaris were the cultivation substrate by Chenopodium quinoa is better than other cereals.
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